蔡少正 教授兼所長

成功大學學士，1986
美國東密西根大學碩士，1992
美國威斯康辛大學博士，1997
加入成功大學教職，1998

email: seantsai@mail.ncku.edu.tw

蔡少正老師實驗室成員

研究方向及興趣：

本實驗室利用生物資訊學及細胞/分生的技術，從事與臨床疾病相關之生理及病理的探討。目前主要的研究方向有三個重點：一、探討子宮內膜異位(endometriosis)成因之分子機制，子宮內膜異位症約佔生殖年齡期婦女人數的10-15%，大部分會導致不孕症的發生，目前有關子宮內膜異位形成之原因，並不清楚。本實驗室過去幾年的研究，在這個領域已有相當不錯的成果，未來將著重在整合性基因功能上的研究；二、缺氧誘導因子(HIF-1a)調控基因與疾病之研究。細胞因為缺氧會產生種種不同的生理反應或病理變化，包括學習記憶、癌症、心血管疾病、生長遲緩、腦部疾病、肺部疾病等。我們利用生物資訊學的方法，將基因體中會受缺氧誘導因子調控之基因（包括人類、大鼠、小鼠）找尋出來，並研究其在生理、病理上的功能；三、研究整個基因體的調控及細胞分化、去分化及在分化的機轉。基因體外調控（epigenomic modification and reprograming）對細胞之命運影響至巨，甚或造成細胞病變。我們利用生物資訊學、功能性基因體學、系統生物學等尖端方法，對此一現象作深入的研究。

1998:國科會研究獎(甲種)

2003:成大醫學中心基礎醫學研究獎

2005-2008:國科會優秀主持人獎

網路教學

Shaw-Jenq Tsai

B.S. National Cheng Kung University, 1986
M.S. Eastern Michigan University, 1992
Ph.D. University of Wisconsin-Madison, 1997
Joined Cheng Kung University faculty, 1998

RESEARCH INTERESTS:

The research interests in my laboratory are primarily focused on three different areas: Investigation of molecular mechanisms leading to the development of endometriosis, hypoxia and human diseases, and application of Bioinformatics and Systems Biology methodologies to study epigenomic modification and genome reprogramming. We employ state-of-the-art techniques and "dry-bench" approaches to carry out these projects.

HOBBIES: Appreciate everything alive.

SPECIALTY:

Molecular Endocrinology;
Cell and Molecular Biology
Bioinformatics

PUBLICATIONS :

• Tsai SJ, Kot K, Ginther OJ, and Wiltbank MC 2001 Real time expression of steady-state concentrations of 9 specific mRNA in bovine corpora lutea after induced luteolysis by prostaglandin F2α Reproduction 121:905-913.
• Tsai SJ* and Wiltbank MC 2001 Differential effects of prostaglandin F2α on in vitro luteinized bovine granulosa cells. Reproduction 122 245-253.
• Anderson LE, Wu YL, Tsai SJ, Wiltbank MC 2001 Prostaglandin F2α receptor in the corpus luteum: Recent information on the gene, messenger ribonucleic acid, and protein. Biol Reprod 64:1041-1047.
• Tsai SJ*, Wu MH, Chuang PC and Chen SM 2001 Distinct regulation of gene expression by prostaglandin F2α (PGF2α) is associated with PGF2α-resistance or susceptibility in human granulosa-lutein cells. Mol Hum Reprod 7:415-423.
• Leung PS, Tsai SJ, Wallukat G, Leung TN, and Lau TK 2001 The upregulation of angiotensin II receptors in human preeclamptic placenta. Mol Cell Endocrinol 184:95-102.
• Lin YM, Chen CW, Sun HS, Tsai SJ, Hsu CC, Teng YN, Lin JS, Kuo PL 2001 Expression patterns and transcript concentrations of the autosomal DAZL gene in testes of azoospermic men. Mol Hum Reprod 7(11):1015-1022.
• Tsai SJ*, Wu MH, Lin CC, Sun HS, and Chen SM 2001 Regulation of steroidogenic acute regulatory protein expression and progesterone production in endometriotic stromal cells. J Clin Endocrinol Metab 86:5765-5773.
• Huang BM, Hsu CC, Tsai SJ, and Leu SF 2001 Effect of Cordyceps sinensis on steroidogenesis in normal mouse Leydig cells. Life Sci 69(22) 2593-2602.
• Wu MH, Sun HS, Lin CC, Hsiao KY, Chuang PC, Pan HA, and Tsai SJ* 2002 Distinct mechanisms regulate cyclooxygenase-1 and -2 in peritoneal macrophages of women with and without endometriosis. Mol Hum Reprod 8:1103-1110.
• Wu MH, Chuang PC, Chen SM, and Tsai SJ* 2002 Expression of leptin in endometriotic cells is associated with endometrial stromal cell proliferation and leptin gene upregulation. Mol Hum Reprod 8:456-464.
• HA Pan, Tsai SJ, Chen CW, Lee YC, Lin YM, Kuo PL 2002 Expression of DAZL protein in human corpus luteum. Mol Hum Reprod 8:540-545.
• Tsai SJ, Wu MH, Chen SM, Chuang PC, and Wing LC 2002 Fibroblast growth factor-9 is an endometrial stromal growth factor. Endocrinology 143:2715-2721.
• Wu MH, Tsai SJ, Pan HA, ShauKY, and Chang FM 2003 Three-dimensional power Doppler imaging of ovarian stromal blood flow in women with endometriosis undergoing in vitro fertilization. Ultrasound Obstet Gynecol 21:480-485.
• Vasconcelos JLM, Sangsritavong S, Tsai SJ, Wiltbank MC. 2003 Acute reduction in serum progesterone concentrations after feed intake in dairy cows. Theriogenology 60: 795-807
• Hsu CC, Huang YL, Tsai SJ, Sheu CC, Huang BM 2003 In vivo and in vitro stimulatory effects of Cordyceps sinensis on testosterone production in mouse Leydig cells. Life Sciences 73:2127-2136.
• SunHS, HsiaoKY, Hsu CC, Wu MH, and Tsai SJ* 2003 Transactivation of steroidogenic acute regulatory protein in human endometriotic stromal cells is mediated by the prostaglandin EP2 receptor. Endocrinology 144:3934-3942
• Hsu CC, Tsai SJ#, Huang YL, and Huang BM 2003 Regulatory mechanism of Cordyceps sinensis mycelium on mouse Leydig cell steroidogenesis.FEBS Lett 543:140-143.
• Wing LC, Chuang PC, Wu MH, Chen HM, and Tsai SJ* 2003 Expression and mitogenic effect of fibroblast growth factor -9 in human endometriotic implant is regulated by aberrant production of estrogen. J Clin Endocrinol Metab88: 5547-5554
• Huang BM, Hsiao KY, Chuang PC, Wu MH, Pan HA, and Tsai SJ*Upregulation of steroidogenic enzymes and ovarian 17b-estradiol in human granulosa-lutein cells by Cordyceps sinensis mycelium Biol Reprod 70:1358-1364.
• Tsai SJ, Lin SJ, Cheng YM, Wing LYC 2005 Expression and functional analysis of pituitary tumor transforming growth factor-1 in uterine leiomyoma J Clin Endocrinol Metab 90:3715-3723
• Wing LYC, Chen SM, Chuang PC, Wu MH, Tsai SJ* 2005 The mTOR-S6K1 but not PI3K-Akt signaling is responsible for fibroblast growth factor-9-induced cell proliferation J Biol Chem 280: 19937-19947
• Chen WC, Chen GY, Tsai SJ, Wnag PW, Sheu HM, Shen YS, Chen FF 2005 Mild cutaneous manifestation in two young women with extraordinary hyperandrogenemia. Dermatology 210:49-52.
• Wu MH, Wang CA, Lin CC, Chen LC, Chang WC, and Tsai SJ* 2005 Distinct Regulation of Cyclooxygenase-2 by Interleukin-1b in Normal and Endometriotic Stromal Cells. J Clin Endocrinol Metab 90: 286-295.
• Wu MH, Shoji Y, Wu MC, Chuang PC, Lin CC, Huang MF, Tsai SJ* 2005 Suppression of matrix metalloproteinase-9 by prostaglandin E2 in peritoneal macrophage is associated with severity of endometriosis. Am J Pathol 167:1061-1069
• Chen KF, Lai YY, Sun HS, and Tsai SJ* 2005 Transcriptional repression of human cad gene by hypoxia inducible factor-1a. Nucleic Acids Research 33:5190-5198
• Chen YF, Kou PL, Tsai SJ, Chen HH, Chen CM, Sun HS 2006 Computational analysis and refinement of sequence structure on Chromosome 22q12.2 region: application to the development of quantitative real-time PCR assey for clinical diagnosis. Genomics 87:290-297.